![]() Antimicrobial resistance is highly prevalent in C. perfringens that targets the plasma membrane leading to hydrolysis of host erythrocytes. It causes numerous diseases with the help of many virulence factors, including its toxins, particularly the alpha toxin (CPA), which is produced by all types of C. perfringens-associated diseases.Ĭlostridium perfringens is the most prevalent spore-forming pathogen of humans and livestock animals. An in vivo approach is the next step to provide evidence for applying these alternatives in the treatment and prevention of C. This is an in vitro study reporting the antimicrobial potential of MYR-coated ZnO nanocomposite as an effective therapeutic candidate against C. Transcriptional modifications of MYR, ZnO, ZnO/PVA, and ZnO/PVA/MYR nanocomposite were determined, and the results showed significant down-regulation of α-hemolysin fold change to 0.5, 0.7, 0.6, and 0.28, respectively compared to the untreated bacteria. Moreover, the MYR either alone or combined with the nanocomposite had no cytotoxic activities on chicken red blood cells (cRBCs). perfringens isolates were susceptible to MYR-loaded ZnO/PVA with minimum inhibitory concentrations (MICs) ranged from 0.125 to 2 µg/mL. The results of broth microdilution technique revealed that all tested C. perfringens isolates were pandrug-resistant (PDR), 4% were extensive drug-resistant (XDR), while 91% were multidrug-resistant. perfringens isolates exhibited high resistance levels for metronidazole (93%), bacitracin (89%), penicillin G (84%), and lincomycin (76%). The antimicrobial susceptibility testing revealed that C. perfringens was higher in chicken (38.3%) than in beef meat products (10%). ![]() perfringens isolated from beef meat and chicken sources. The current study was performed to investigate the inhibitory activity of myricetin (MYR), polyvinyl alcohol (PVA), and zinc oxide (ZnO) nanocomposite against growth and α-hemolysin of C. perfringens) is an important pathogen in livestock animals and humans causing a wide array of systemic and enteric diseases. ![]()
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